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Pyruvate Dehydrogenase E1 alpha antibody

GTX104015
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetPDHA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Pyruvate Dehydrogenase E1 alpha antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.39 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5160
  • Target name
    PDHA1
  • Target description
    pyruvate dehydrogenase E1 subunit alpha 1
  • Target synonyms
    PDHA; PDHAD; PDHCE1A; PDHE1-A type I; PHE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08559
  • Protein Name
    Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
  • Scientific Description
    The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Trehalose enhances mitochondria deficits in human NPC1 mutant fibroblasts but disrupts mouse Purkinje cell dendritic growth ex vivo.
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  • Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease.
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  • A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
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  • Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue. Rosina M et al., 2022 Apr 5, Cell Metab
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  • Selective knockdown of hexokinase 2 in rods leads to age-related photoreceptor degeneration and retinal metabolic remodeling. Zhang R et al., 2020 Oct 20, Cell Death Dis
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  • Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. Rey T et al., 2020 Oct, Nat Cell Biol
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  • Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy. Jonus HC et al., 2020 Jan, Biomed Pharmacother
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  • Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris. Chen Y et al., 2015 Sep 25, J Biol Chem
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  • Protein kinase B (PKB/AKT1) formed signaling complexes with mitochondrial proteins and prevented glycolytic energy dysfunction in cultured cardiomyocytes during ischemia-reperfusion injury. Deng W et al., 2014 May, Endocrinology
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  • Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization. Chao LC et al., 2012 Dec, J Lipid Res
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