QTRT1 Antibody

Cusabio

QTRT1 Antibody

20

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

Unconjugated

QTRT1

queuine tRNA-ribosyltransferase catalytic subunit 1

FP3235; guanine insertion enzyme; queuine tRNA-ribosyltransferase 1; queuine tRNA-ribosyltransferase catalytic subunit 1; TGT; TGT, 43-KD subunit; TGT, catalytic subunit; TGUT; tRNA-guanine transglycosylase

Rabbit

IgG

Queuine tRNA-ribosyltransferase catalytic subunit 1

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1 of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1 of the ribose to form the product (By similarity).

-20°C or -80°C

12352203