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ICC/IF analysis of LOVO cells using GTX82800 Rab10 antibody [4E2]. Green : Rab10 Blue: DRAQ5 fluorescent DNA dye
ICC/IF analysis of LOVO cells using GTX82800 Rab10 antibody [4E2]. Green : Rab10 Blue: DRAQ5 fluorescent DNA dye
ICC/IF analysis of LOVO cells using GTX82800 Rab10 antibody [4E2]. Green : Rab10 Blue: DRAQ5 fluorescent DNA dye

Rab10 antibody [4E2]

GTX82800
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetRAB10
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rab10 antibody [4E2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    400
  • Conjugate
    Unconjugated
  • Gene ID10890
  • Target name
    RAB10
  • Target description
    RAB10, member RAS oncogene family
  • Target synonyms
    GTP-binding protein RAB10; ras-related GTP-binding protein; ras-related protein Rab-10
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP61026
  • Protein Name
    Ras-related protein Rab-10
  • Scientific Description
    RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Direct lysosome-based autophagy of lipid droplets in hepatocytes. Schulze RJ et al., 2020 Dec 22, Proc Natl Acad Sci U S A
    Read more
  • IKKbeta activation promotes amphisome formation and extracellular vesicle secretion in tumor cells. Peng X et al., 2021 Jan, Biochim Biophys Acta Mol Cell Res
    Read more
  • Maturation of Lipophagic Organelles in Hepatocytes Is Dependent Upon a Rab10/Dynamin-2 Complex. Li Z et al., 2020 Aug, Hepatology
    Read more
  • A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Li Z et al., 2016 Dec, Sci Adv
    Read more

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Figure 1. Western blot analysis of RAB10 using anti-RAB10 antibody (PB9788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (Catalog # PB9788) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 23 kDa. The expected band size for RAB10 is at 23 kDa.
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