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Rab10 Antibody (YA1789)

HY-P82044
MedChem Express
TargetRAB10
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Rab10 Antibody (YA1789)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10890
  • Target name
    RAB10
  • Target description
    RAB10, member RAS oncogene family
  • Target synonyms
    GTP-binding protein RAB10; ras-related GTP-binding protein; ras-related protein Rab-10
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rab10 Antibody (YA1789) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1789), targeting Rab10, with a predicted molecular weight of 23 kDa (observed band size: 23 kDa). Rab10 Antibody (YA1789) can be used for WB, ICC/IF, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB10 using anti-RAB10 antibody (PB9788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (Catalog # PB9788) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 23 kDa. The expected band size for RAB10 is at 23 kDa.
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