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RAB11B Antibody

CSB-PA070266
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAB11B
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB11B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9230
  • Target name
    RAB11B
  • Target description
    RAB11B, member RAS oncogene family
  • Target synonyms
    H-YPT3, NDAGSCW, ras-related protein Rab-11B, GTP-binding protein YPT3, RAB11B, member of RAS oncogene family
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15907
  • Protein Name
    Ras-related protein Rab-11B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # A04526-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.
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