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Western Blot analysis of MCF-7 cells using Rab 18 Polyclonal Antibody
Western Blot analysis of MCF-7 cells using Rab 18 Polyclonal Antibody
Western Blot analysis of MCF-7 cells using Rab 18 Polyclonal Antibody

RAB18 Antibody

CSB-PA008282
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAB18
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22931
  • Target name
    RAB18
  • Target description
    RAB18, member RAS oncogene family
  • Target synonyms
    RAB18 small GTPase; RAB18LI1; ras-related protein Rab-18; WARBM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP72
  • Protein Name
    Ras-related protein Rab-18
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: PC-3 Cell lysates; Lane 2: mouse brain lysates; Lane 3: rat brain lysates; Lane 4: human brain lysates; Lane 5: human testis lysates; probed with RAB18 (3A8) Monoclonal Antibody, unconjugated (bsm-51333M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of Rab18 using anti-Rab18 antibody (PB9791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: 293T Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab18 antigen affinity purified polyclonal antibody (Catalog # PB9791) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab18 at approximately 23 kDa. The expected band size for Rab18 is at 23 kDa.
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