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Western blot All lanes: RAB23 antibody at 3.88ug/ml Lane 1: Mouse ocular tissue Lane 2: Rat brain tissue Lane 3: Mouse kidney tissue Lane 4: Mouse lung tissue Lane 5: Mouse brain tissue Lane 6: NIH/3T3 whole cell lysate Lane 7: MCF-7 whole cell lysate Lane 8: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa
Western blot All lanes: RAB23 antibody at 3.88ug/ml Lane 1: Mouse ocular tissue Lane 2: Rat brain tissue Lane 3: Mouse kidney tissue Lane 4: Mouse lung tissue Lane 5: Mouse brain tissue Lane 6: NIH/3T3 whole cell lysate Lane 7: MCF-7 whole cell lysate Lane 8: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa
Western blot All lanes: RAB23 antibody at 3.88ug/ml Lane 1: Mouse ocular tissue Lane 2: Rat brain tissue Lane 3: Mouse kidney tissue Lane 4: Mouse lung tissue Lane 5: Mouse brain tissue Lane 6: NIH/3T3 whole cell lysate Lane 7: MCF-7 whole cell lysate Lane 8: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa

RAB23 Antibody

CSB-PA891960ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAB23
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB23 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51715
  • Target name
    RAB23
  • Target description
    RAB23, member RAS oncogene family
  • Target synonyms
    HSPC137; RAB family small GTP binding protein RAB 23; ras-related protein Rab-23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULC3
  • Protein Name
    Ras-related protein Rab-23
  • Scientific Description
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB23 using anti-RAB23 antibody (A04593-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB23 antigen affinity purified polyclonal antibody (Catalog # A04593-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB23 at approximately 27 kDa. The expected band size for RAB23 is at 27 kDa.
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