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ProductsBack/RAB27A Rabbit Polyclonal Antibody
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: K562 cells Primary antibody: TA364951 (RAB27A Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: K562 cells Primary antibody: TA364951 (RAB27A Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: K562 cells Primary antibody: TA364951 (RAB27A Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

RAB27A Rabbit Polyclonal Antibody

TA364951
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetRAB27A
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RAB27A Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5873
  • Target name
    RAB27A
  • Target description
    RAB27A, member RAS oncogene family
  • Target synonyms
    GS2; GTP-binding protein Ram; HsT18676; mutant Ras-related protein Rab-27A; RAB27; rab-27; RAM; ras-related protein Rab-27A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51159
  • Protein Name
    Ras-related protein Rab-27A
  • Scientific Description
    RAB27A rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB27A using anti-RAB27A antibody (A01608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat stomach tissue lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27A antigen affinity purified polyclonal antibody (Catalog # A01608-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 25KD.
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