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ProductsBack/RAB3A Rabbit Polyclonal Antibody
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-3: Human cerebella tissue Human cerebrum tissue and Mouse brain tissue lysates Primary antibody: TA364950 (RAB3A Antibody) at dilution 1/900 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-3: Human cerebella tissue Human cerebrum tissue and Mouse brain tissue lysates Primary antibody: TA364950 (RAB3A Antibody) at dilution 1/900 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-3: Human cerebella tissue Human cerebrum tissue and Mouse brain tissue lysates Primary antibody: TA364950 (RAB3A Antibody) at dilution 1/900 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

RAB3A Rabbit Polyclonal Antibody

TA364950
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetRAB3A
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RAB3A Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5864
  • Target name
    RAB3A
  • Target description
    RAB3A, member RAS oncogene family
  • Target synonyms
    RAS-associated protein RAB3A; ras-related protein Rab-3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20336
  • Protein Name
    Ras-related protein Rab-3A
  • Scientific Description
    RAB3A rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Rab3A using anti-Rab3A antibody (PB9322). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab3A antigen affinity purified polyclonal antibody (Catalog # PB9322) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab3A at approximately 25 kDa. The expected band size for Rab3A is at 25 kDa.
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