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Rabbit anti-Alpha-4 Antibody, Affinity Purified

A300-470A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetIGBP1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Alpha-4 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3476
  • Target name
    IGBP1
  • Target description
    immunoglobulin binding protein 1
  • Target synonyms
    ALPHA-4, IBP1, MRXS28, alpha4, immunoglobulin-binding protein 1, B cell signal transduction molecule alpha 4, CD79a-binding protein 1, bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ), immunoglobulin (CD79A) binding protein 1, protein alpha-4, protein phosphatase 2/4/6 regulatory subunit, protein phosphatase 2A, regulatory subunit alpha-4, renal carcinoma antigen NY-REN-16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78318
  • Protein Name
    Immunoglobulin-binding protein 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IGBP1 using anti-IGBP1 antibody (A05930-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGBP1 antigen affinity purified polyclonal antibody (Catalog # A05930-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGBP1 at approximately 45 kDa. The expected band size for IGBP1 is at 45 kDa.
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