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Rabbit anti-B-Myb Antibody, Affinity Purified

A301-654A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetMYBL2
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-B-Myb Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4605
  • Target name
    MYBL2
  • Target description
    MYB proto-oncogene like 2
  • Target synonyms
    B-MYB, BMYB, myb-related protein B, myb-like protein 2, v-myb avian myeloblastosis viral oncogene homolog-like 2, v-myb myeloblastosis viral oncogene homolog-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10244
  • Protein Name
    Myb-related protein B
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of B MyB/MYBL2 using anti-B MyB/MYBL2 antibody (A02597). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B MyB/MYBL2 antigen affinity purified polyclonal antibody (Catalog # A02597) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B MyB/MYBL2 at approximately 100KD. The expected band size for B MyB/MYBL2 is at 100KD.
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