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Rabbit anti-CBX5 Antibody, Affinity Purified

A300-877A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCBX5
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CBX5 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23468
  • Target name
    CBX5
  • Target description
    chromobox 5
  • Target synonyms
    HEL25, HP1, HP1A, HP1alpha, chromobox protein homolog 5, HP1 alpha homolog, HP1-ALPHA, HP1Hs alpha, antigen p25, chromobox homolog 5 (HP1 alpha homolog, Drosophila), epididymis luminal protein 25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45973
  • Protein Name
    Chromobox protein homolog 5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (A02780-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 alpha/CBX5 antigen affinity purified polyclonal antibody (Catalog # A02780-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 alpha/CBX5 at approximately 22KD. The expected band size for HP1 alpha/CBX5 is at 22KD.
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