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ProductsBack/Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR233K]

Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR233K]

A700-233
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCd79a
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR233K]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR233K
  • Concentration
    250 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID12518
  • Target name
    Cd79a
  • Target description
    CD79A antigen (immunoglobulin-associated alpha)
  • Target synonyms
    B-cell antigen receptor complex-associated protein alpha chain; Cd79; I; Ig; Ig-; Iga; Igalpha; Ig-alpha; Ly5; Ly-5; Ly54; Ly-54; mb-1; MB-1 membrane glycoprotein; membrane-bound immunoglobulin-associated protein; surface IgM-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11911
  • Protein Name
    B-cell antigen receptor complex-associated protein alpha chain
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd79a using anti-Cd79a antibody (A01047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd79a antigen affinity purified polyclonal antibody (Catalog # A01047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd79a at approximately 25-30 kDa. The expected band size for Cd79a is at 25 kDa.
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