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ProductsBack/Rabbit anti-CD80 Recombinant Monoclonal Antibody [BLR206K]

Rabbit anti-CD80 Recombinant Monoclonal Antibody [BLR206K]

A700-206
Bethyl Laboratories
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD80
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD80 Recombinant Monoclonal Antibody [BLR206K]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR206K
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID941
  • Target name
    CD80
  • Target description
    CD80 molecule
  • Target synonyms
    B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, B-lymphocyte activation antigen B7, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CTLA-4 counter-receptor B7.1, activation B7-1 antigen, costimulatory factor CD80, costimulatory molecule variant IgV-CD80
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33681
  • Protein Name
    T-lymphocyte activation antigen CD80
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD80 using anti-CD80 antibody (A00196-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # A00196-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD80 at approximately 30-70 kDa. The expected band size for CD80 is at 33 kDa.
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