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ProductsBack/Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]

Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]

ORB1566957
Biorbyt
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCeacam1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]
  • Delivery Days Customer
    10
  • Application Supplier Note
    Format: Whole IgG. WB - 1:1,000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. FC, ICC, IHC, IP, WB
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    50 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID26365
  • Target name
    Ceacam1
  • Target description
    CEA cell adhesion molecule 1
  • Target synonyms
    Bgp, Bgp1, C-CAM, CD66a, Cc1, Cea-1, Cea-7, Cea1, Cea7, Hv-2, Hv2, MHVR, MHVR1, Mhv-1, bb-1, mCEA1, mmCGM1, mmCGM1a, mmCGM2, cell adhesion molecule CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1, BGP-1, CEA-related cell adhesion molecule 1, MHV-R, biliary glycoprotein 1, biliary glycoprotein D, carcinoembryonic antigen 1, carcinoembryonic antigen 7, hepatitis virus (MHV-4) susceptibility, hepatitis virus receptor, murine hepatitis virus receptor
  • Host
    Rabbit
  • Protein IDP31809
  • Protein Name
    Cell adhesion molecule CEACAM1
  • Scientific Description
    CEACAM1 Antibody
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Raw264.7 whole cell lysates, Lane 3: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 150 kDa. The expected band size for CEACAM1 is at 57,50,37,30 kDa.
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