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Rabbit anti-DDX1 Antibody, Affinity Purified

A300-520A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Primate, Rat
TargetDDX1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-DDX1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1653
  • Target name
    DDX1
  • Target description
    DEAD-box helicase 1
  • Target synonyms
    DBP-RB, UKVH5d, ATP-dependent RNA helicase DDX1, DEAD (Asp-Glu-Ala-Asp) box helicase 1, DEAD (Asp-Glu-Ala-Asp) box polypeptide 1, DEAD box polypeptide 1, DEAD box protein 1, DEAD box protein retinoblastoma, DEAD box-1, DEAD-box RNA helicase DDX1, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1, DEAD/H-box helicase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92499
  • Protein Name
    ATP-dependent RNA helicase DDX1
  • Reactivity
    Bovine, Human, Mouse, Primate, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, fallopian tube, kidney and skeletal muscle using Anti-DDX1 antibody HPA034502 (A) shows similar protein distribution across tissues to independent antibody HPA034503 (B).
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Figure 1. Western blot analysis of DDX1 using anti-DDX1 antibody (A03727-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX1 antigen affinity purified polyclonal antibody (Catalog # A03727-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX1 at approximately 88 kDa. The expected band size for DDX1 is at 82 kDa.
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