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Rabbit anti-DDX21 Antibody, Affinity Purified

A300-627A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetDDX21
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-DDX21 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9188
  • Target name
    DDX21
  • Target description
    DExD-box helicase 21
  • Target synonyms
    GUA, GURDB, II/Gu, RH, RH II/Gu, RH-II/GU, RH-II/GuA, gu-alpha, nucleolar RNA helicase 2, DEAD (Asp-Glu-Ala-Asp) box helicase 21, DEAD box protein 21, DEAD-box helicase 21, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21, Gu protein, RNA helicase II/Gu alpha, nucleolar RNA helicase Gu, nucleolar RNA helicase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR30
  • Protein Name
    Nucleolar RNA helicase 2
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDX21 using anti-DDX21 antibody (A04605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX21 antigen affinity purified polyclonal antibody (Catalog # A04605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX21 at approximately 86 kDa. The expected band size for DDX21 is at 86 kDa.
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