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ProductsBack/Rabbit anti-ERCC3 Antibody, Affinity Purified

Rabbit anti-ERCC3 Antibody, Affinity Purified

A301-336A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetERCC3
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-ERCC3 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2071
  • Target name
    ERCC3
  • Target description
    ERCC excision repair 3, TFIIH core complex helicase subunit
  • Target synonyms
    BTF2, GTF2H, RAD25, Ssl2, TFIIH, TTD2, XPB, general transcription and DNA repair factor IIH helicase/translocase subunit XPB, BTF2 p89, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH p89, TFIIH subunit XPB, basic transcription factor 2 89 kDa subunit, excision repair cross-complementation group 3, excision repair cross-complementing rodent repair deficiency, complementation group 3, general transcription and DNA repair factor IIH helicase subunit XPB, xeroderma pigmentosum group B-complementing protein, xeroderma pigmentosum, complementation group B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19447
  • Protein Name
    General transcription and DNA repair factor IIH helicase subunit XPB
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XPB using anti-XPB antibody (A03103-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPB antigen affinity purified polyclonal antibody (Catalog # A03103-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPB at approximately 89KD. The expected band size for XPB is at 89KD.
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