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Rabbit anti Human Caspase-7

A135P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetCASP7
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Caspase-7
  • Delivery Days Customer
    7
  • Application Supplier Note
    Applications: western blot, use at 2-10 microg/ml
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID840
  • Target name
    CASP7
  • Target description
    caspase 7
  • Target synonyms
    apoptotic protease MCH-3; CASP-7; caspase 7, apoptosis-related cysteine peptidase; caspase 7, apoptosis-related cysteine protease; caspase-7; CMH-1; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55210
  • Protein Name
    Caspase-7
  • Scientific Description
    Cysteine-requiring Aspartate Protease-7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Rabbits with the aa 186-198 of human caspase-7
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-7/CASP7 using anti-Caspase-7/CASP7 antibody (A01044-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-7/CASP7 antigen affinity purified polyclonal antibody (Catalog # A01044-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-7/CASP7 at approximately 35KD. The expected band size for Caspase-7/CASP7 is at 35KD.
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