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ProductsBack/Anti-FLT-1 / VEGFR1 (C-term) Antibody

Anti-FLT-1 / VEGFR1 (C-term) Antibody

119-11310
RayBiotech
TargetFLT1
Product group Antibodies
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Rabbit Anti-Human FLT-1 / VEGFR1 (C-Terminus)
  • Delivery Days Customer
    4
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2321
  • Target name
    FLT1
  • Target description
    fms related receptor tyrosine kinase 1
  • Target synonyms
    FLT; FLT-1; fms related tyrosine kinase 1; fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; vascular permeability factor receptor; VEGFR1; VEGFR-1
  • Host
    Rabbit
  • Protein IDP17948
  • Protein Name
    Vascular endothelial growth factor receptor 1
  • Scientific Description
    FLT-1 / VEGFR1 Rabbit anti-Human Polyclonal (C-Terminus) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 150KD. The expected band size for VEGF Receptor 1 is at 150KD.
Product group Antibodies
References
Boster Bio
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Biomatik
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Mouse lung lysates probed with VEGF Receptor 1 (3A6) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Bioss Antibodies
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