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Mouse lung lysates probed with VEGF Receptor 1 (3A6) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse lung lysates probed with VEGF Receptor 1 (3A6) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse lung lysates probed with VEGF Receptor 1 (3A6) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

VEGFR1 Recombinant Antibody

BSM-52338R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFLT1
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Overview

  • Supplier
    Bioss
  • Product Name
    VEGF Receptor 1 (3A6) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2321
  • Target name
    FLT1
  • Target description
    fms related receptor tyrosine kinase 1
  • Target synonyms
    FLT, FLT-1, VEGFR-1, VEGFR1, vascular endothelial growth factor receptor 1, fms related tyrosine kinase 1, fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor), tyrosine-protein kinase FRT, tyrosine-protein kinase receptor FLT, vascular permeability factor receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17948
  • Protein Name
    Vascular endothelial growth factor receptor 1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 150KD. The expected band size for VEGF Receptor 1 is at 150KD.
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