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Anti-GCP2 (N-term) Antibody

107-10332
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTUBGCP2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GCP2 (N-term) Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID10844
  • Target name
    TUBGCP2
  • Target description
    tubulin gamma complex component 2
  • Target synonyms
    ALP4, GCP-2, GCP2, Grip103, PAMDDFS, SPBC97, SPC97, Spc97p, h103p, hGCP2, hSpc97, gamma-tubulin complex component 2, gamma-ring complex protein 103 kDa, hGrip103, spindle pole body protein Spc97 homolog, tubulin gamma complex associated protein 2
  • Host
    Rabbit
  • Scientific Description
    GCP2 antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TUBGCP2 using anti-TUBGCP2 antibody (A10573-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBGCP2 antigen affinity purified polyclonal antibody (Catalog # A10573-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBGCP2 at approximately 103 kDa. The expected band size for TUBGCP2 is at 103 kDa.
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