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ProductsBack/Rabbit anti Human Histone H2A.x (pS140)

Rabbit anti Human Histone H2A.x (pS140)

X2752P
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetH2AX
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Histone H2A.x (pS140)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1-5 microg/ml) and immunohistochemistry (2-5 microg/ml at pH 6.2). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Enzyme Immunoassay;Western Blotting;Immunohistochemistry;Enzyme Immunoassay;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Gene ID3014
  • Target name
    H2AX
  • Target description
    H2A.X variant histone
  • Target synonyms
    H2A histone family member X; H2A.X; H2A/X; H2AFX; H2AX histone; histone H2A.x; histone H2AX
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16104
  • Protein Name
    Histone H2AX
  • Scientific Description
    H2AFX; H2AX
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Phosphopeptide derived from the human histone H2A.x protein.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of H2AFX using anti-H2AFX antibody (A00241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified polyclonal antibody (Catalog # A00241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.
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