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Anti-MIA3 (N-term) Antibody

107-10292
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMIA3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Rabbit Anti-Human MIA3, N-term
  • Delivery Days Customer
    4
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Concentration
    0.3 mg/ml
  • Gene ID375056
  • Target name
    MIA3
  • Target description
    MIA SH3 domain ER export factor 3
  • Target synonyms
    ARNT; C219-reactive peptide; D320; melanoma inhibitory activity family, member 3; melanoma inhibitory activity protein 3; MIA family member 3, ER export factor; ODCD2; TANGO; TANGO1; transport and Golgi organization protein 1 homolog; UNQ6077
  • Host
    Rabbit
  • Scientific Description
    MIA3 antibody [N1], N-Terminus, 0.3 mg/ml (100 ul)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIA3 using anti-MIA3 antibody (A06158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIA3 antigen affinity purified polyclonal antibody (Catalog # A06158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIA3 at approximately 300 kDa. The expected band size for MIA3 is at 300 kDa.
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