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ProductsBack/Rabbit anti Human Protein Phosphatase 2 B alpha (calcineurin)

Rabbit anti Human Protein Phosphatase 2 B alpha (calcineurin)

P195P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPPP3CA
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Protein Phosphatase 2 B alpha (calcineurin)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Western Blot,Immunohistochemistry
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    Immunocytochemistry;Western Blotting;Immunohistochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5530
  • Target name
    PPP3CA
  • Target description
    protein phosphatase 3 catalytic subunit alpha
  • Target synonyms
    ACCIID; calcineurin A alpha; CALN; CALNA; CALNA1; CAM-PRP catalytic subunit; CCN1; CNA1; DEE91; IECEE; IECEE1; PPP2B; protein phosphatase 3 catalytic subunit alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08209
  • Protein Name
    Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
  • Scientific Description
    pp2Ba, rabbit anti protein phosphatase 2 B alpha calcineurin; protein phosphatase 3
  • Shelf life instruction
    See expiration date on vial
  • Source
    The immunogen used for the anti-protein phosphatase 2B was a purified peptide conjugated to KLH. The sequence of the peptide is NH2-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-Ser-Asp-Ala-COOH (a.a. 482-494) corresponding to a 60kDa protein
  • Reactivity Supplier
    Human;Mouse;Rat;Bovine
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP3CA using anti-PPP3CA antibody (A03026-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CA antigen affinity purified polyclonal antibody (Catalog # A03026-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CA at approximately 59 kDa. The expected band size for PPP3CA is at 59 kDa.
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