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Anti-RORB / ROR Beta (Hinge Domain) Antibody

119-10197
RayBiotech
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRORB
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Rabbit Anti-Human RORB / ROR Beta (Hinge Domain)
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID6096
  • Target name
    RORB
  • Target description
    RAR related orphan receptor B
  • Target synonyms
    bA133M9.1; EIG15; NR1F2; nuclear receptor ROR-beta; nuclear receptor RZR-beta; nuclear receptor subfamily 1 group F member 2; RAR-related orphan receptor beta; retinoic acid-binding receptor beta; retinoid-related orphan receptor beta; ROR-BETA; RZRB; RZR-BETA
  • Host
    Rabbit
  • Protein IDQ92753
  • Protein Name
    Nuclear receptor ROR-beta
  • Scientific Description
    RORB / ROR Beta Rabbit anti-Human Polyclonal (Hinge Domain) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse Eye cell lysates; Lane 2: Rat Eye cell lysates; Lane 3: Mouse Cerebrum cell lysates; Lane 4: Rat Cerebrum cell lysates ; Lane 5: Mouse Uterus cell lysates probed with ROR beta Polyclonal Antibody, Unconjugated (bs-6216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ROR beta/RORB using anti-ROR beta/RORB antibody (A09711-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR beta/RORB antigen affinity purified polyclonal antibody (Catalog # A09711-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR beta/RORB at approximately 53 kDa. The expected band size for ROR beta/RORB is at 53 kDa.
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