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Anti-Sec61 gamma subunit Antibody

107-10015
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSEC61G
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Sec61 gamma subunit Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID23480
  • Target name
    SEC61G
  • Target description
    SEC61 translocon subunit gamma
  • Target synonyms
    SSS1, protein transport protein Sec61 subunit gamma, SEC61 translocon gamma subunit, Sec61 gamma subunit, protein transport protein SEC61 gamma subunit
  • Host
    Rabbit
  • Scientific Description
    Sec61 gamma subunit antibody, 1 mg/ml (100 ul)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC61G using anti-SEC61G antibody (A12389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61G antigen affinity purified polyclonal antibody (Catalog # A12389) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC61G at approximately 12 kDa. The expected band size for SEC61G is at 8 kDa.
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