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Rabbit anti Human Sirt1 Protein (Human)

X2739P
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSIRT1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Sirt1 Protein (Human)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Immunohistochemistry;Western Blotting;ELISA;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    >95%
  • Gene ID23411
  • Target name
    SIRT1
  • Target description
    sirtuin 1
  • Target synonyms
    NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; regulatory protein SIR2 homolog 1; SIR2; SIR2alpha; SIR2L1; SIR2-like protein 1; sirtuin type 1
  • Host
    Rabbit
  • Protein IDQ96EB6
  • Protein Name
    NAD-dependent protein deacetylase sirtuin-1
  • Scientific Description
    NAD-dependent deacetylase sirtuin-1, hSIRT1, SIR2-like protein 1, hSIR2, SIRT1, SIR2L1
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Synthetic Peptide near C terminal of human Sirt 1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (Catalog # A00018-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.
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