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ProductsBack/Rabbit anti Human SPT16 NT

Rabbit anti Human SPT16 NT

X1583P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetSSRP1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human SPT16 NT
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6749
  • Target name
    SSRP1
  • Target description
    structure specific recognition protein 1
  • Target synonyms
    FACT, FACT80, T160, FACT complex subunit SSRP1, FACT 80 kDa subunit, FACTp80, chromatin-specific transcription elongation factor 80 kDa subunit, cisplatin-DNA SSRP, facilitates chromatin remodeling 80 kDa subunit, facilitates chromatin transcription complex 80 kDa subunit, facilitates chromatin transcription complex subunit SSRP1, hSSRP1, high mobility group box, recombination signal sequence recognition protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08945
  • Protein Name
    FACT complex subunit SSRP1
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human;Mouse
  • Reactivity Supplier Note
    Recombinant protein consisting of amino acids 1-329 of the human SPT16 protein conjugated to KLH.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human DLD-1 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysate, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSRP1 antigen affinity purified polyclonal antibody (Catalog # A02606-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSRP1 at approximately 92 kDa. The expected band size for SSRP1 is at 81 kDa.
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