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Rabbit anti-IMMT/Mitofilin Antibody, Affinity Purified

ORB1521876
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetIMMT
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Rabbit anti-IMMT/Mitofilin Antibody, Affinity Purified
  • Delivery Days Customer
    10
  • Application Supplier Note
    Format: Whole IgG
  • Applications
    Western Blot
  • Applications Supplier
    WB - 1:2,000 - 1:10,000; IP - Not recommended WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID10989
  • Target name
    IMMT
  • Target description
    inner membrane mitochondrial protein
  • Target synonyms
    HMP, MICOS60, MINOS2, Mic60, P87, P87/89, P89, PIG4, PIG52, MICOS complex subunit MIC60, cell proliferation-inducing gene 4/52 protein, cell proliferation-inducing protein 52, heart muscle protein, mitochondrial contact site and cristae organizing system subunit 60, mitochondrial inner membrane organizing system 2, mitochondrial inner membrane protein, mitofilin, motor protein, proliferation-inducing gene 4
  • Host
    Rabbit
  • Protein IDQ16891
  • Protein Name
    MICOS complex subunit MIC60
  • Scientific Description
    IMMT/Mitofilin Antibody
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (A04102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse HEPA1/6 whole cell lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (Catalog # A04102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.
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