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Rabbit anti-LOX Antibody

ORB1193004
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLOX
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Rabbit anti-LOX Antibody
  • Delivery Days Customer
    10
  • Applications
    Western Blot
  • Applications Supplier
    WB (1/500 - 1/2000) WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4015
  • Target name
    LOX
  • Target description
    lysyl oxidase
  • Target synonyms
    AAT10, protein-lysine 6-oxidase
  • Host
    Rabbit
  • Protein IDP28300
  • Protein Name
    Protein-lysine 6-oxidase
  • Scientific Description
    Rabbit polyclonal antibody to LOX
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
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