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Rabbit anti-MARK3 Antibody, Affinity Purified

A302-186A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetMARK3
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-MARK3 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4140
  • Target name
    MARK3
  • Target description
    microtubule affinity regulating kinase 3
  • Target synonyms
    CTAK1, KP78, PAR1A, Par-1a, VIPB, MAP/microtubule affinity-regulating kinase 3, C-TAK1, ELKL motif kinase 2, EMK-2, cdc25C-associated protein kinase 1, protein kinase STK10, ser/Thr protein kinase PAR-1, serine/threonine-protein kinase p78
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27448
  • Protein Name
    MAP/microtubule affinity-regulating kinase 3
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MARK3 using anti-MARK3 antibody (A05355-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse EL-4 whole cell lysates, Lane 5: mouse 4T1 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (Catalog # A05355-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.
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