An ELISA plate is coated with 50 μL of a 6x-His recombinant protein conjugated BSA at concentration ranged from 0.003 μg/mL to 3 μg/mL. The coated protein is detected with anti-His antibody (GTX628921) at 0.5 ng/mL. Bound antibodies were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213112-01).
An ELISA plate is coated with 50 μL of a 6x-His recombinant protein conjugated BSA at concentration ranged from 0.003 μg/mL to 3 μg/mL. The coated protein is detected with anti-His antibody (GTX628921) at 0.5 ng/mL. Bound antibodies were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213112-01).
Rabbit Anti-Mouse IgG antibody (HRP)
GTX213112-01
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
Overview
- SupplierGeneTex
- Product NameRabbit Anti-Mouse IgG antibody (HRP)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.48 mg/ml
- ConjugateHRP
- HostRabbit
- IsotypeIgG
- Scientific DescriptionImmunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161