ELISA analysis of different amount of immunogen proteins using serial diluted GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103].
![ELISA analysis of mouse immunoglobulins (50 ng/well) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103]. No cross reactivity with the lamda lightchain, human IgG (kappa+lambda), rat IgG (kappa+lambda), or goat IgG (kappa+lambda). Dilution : 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX60858/GTX60858_20200909_ELISA_65_w_23061123_214.webp "ELISA analysis of mouse immunoglobulins (50 ng/well) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103]. No cross reactivity with the lamda lightchain, human IgG (kappa+lambda), rat IgG (kappa+lambda), or goat IgG (kappa+lambda). Dilution : 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody")
![WB analysis of nonreduced(-) and reduced(+) mouse IgG1kappa and IgG1lambda (20ng/lane) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103]. Dilution : 0.2microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60858/GTX60858_20200909_WB_302_w_23061123_850.webp "WB analysis of nonreduced(-) and reduced(+) mouse IgG1kappa and IgG1lambda (20ng/lane) using GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103]. Dilution : 0.2microg/ml")
ELISA analysis of different amount of immunogen proteins using serial diluted GTX60858 Rabbit Anti-Mouse kappa Light chain antibody [RM103].
Rabbit Anti-Mouse kappa Light chain antibody [RM103]
GTX60858
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetIgk
Overview
- SupplierGeneTex
- Product NameRabbit Anti-Mouse kappa Light chain antibody [RM103]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of RM103 has been engineered to eliminate Fc receptor binding.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM103
- ConjugateUnconjugated
- Gene ID243469
- Target nameIgk
- Target descriptionimmunoglobulin kappa chain complex
- Target synonymsk; kappa; kappa light chain variable region
- HostRabbit
- IsotypeIgG
- Scientific DescriptionSummary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Jul 2008]
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203