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ProductsBack/Rabbit anti-Nectin-3 Recombinant Monoclonal Antibody [BLR307M]

Rabbit anti-Nectin-3 Recombinant Monoclonal Antibody [BLR307M]

A700-307
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNECTIN3
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Nectin-3 Recombinant Monoclonal Antibody [BLR307M]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR307M
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25945
  • Target name
    NECTIN3
  • Target description
    nectin cell adhesion molecule 3
  • Target synonyms
    CD113, CDW113, NECTIN-3, PPR3, PRR3, PVRL3, PVRR3, nectin-3, nectin 3, poliovirus receptor-related 3, poliovirus receptor-related protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQS3
  • Protein Name
    Nectin-3
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nectin 3/NECTIN3 using anti-Nectin 3/NECTIN3 antibody (A09633-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. Lane 2: human HEK293 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 3/NECTIN3 antigen affinity purified polyclonal antibody (Catalog # A09633-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 3/NECTIN3 at approximately 80KD. The expected band size for Nectin 3/NECTIN3 is at 61KD.
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