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ProductsBack/Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J]

Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J]

A700-190
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNefh
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR190J
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID380684
  • Target name
    Nefh
  • Target description
    neurofilament, heavy polypeptide
  • Target synonyms
    NF-H, NF200, Nfh, mKIAA0845, neurofilament heavy polypeptide, 200 kDa neurofilament protein, neurofilament 200kDa, neurofilament triplet H protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19246
  • Protein Name
    Neurofilament heavy polypeptide
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nefh using anti-Nefh antibody (A05307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nefh antigen affinity purified polyclonal antibody (Catalog # A05307) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nefh at approximately 117-220KD. The expected band size for Nefh is at 117KD.
Product group Antibodies
Boster Bio
Anti-Nefh Antibody Picoband(r)A05307-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetNefh
  • SizePrice