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ProductsBack/Rabbit anti-NONO Antibody, Affinity Purified

Rabbit anti-NONO Antibody, Affinity Purified

A300-582A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNONO
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NONO Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4841
  • Target name
    NONO
  • Target description
    non-POU domain containing octamer binding
  • Target synonyms
    MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114, non-POU domain-containing octamer-binding protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, non-POU domain-containing octamer (ATGCAAAT) binding protein, p54(nrb), protein phosphatase 1, regulatory subunit 114
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15233
  • Protein Name
    Non-POU domain-containing octamer-binding protein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nmt55/p54nrb antigen affinity purified polyclonal antibody (Catalog # PB9875) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nmt55/p54nrb at approximately 60KD. The expected band size for nmt55/p54nrb is at 60KD.
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