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Rabbit anti-NUDT9 Antibody, Affinity Purified

A305-703A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNUDT9
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NUDT9 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID53343
  • Target name
    NUDT9
  • Target description
    nudix hydrolase 9
  • Target synonyms
    NUDT10, ADP-ribose pyrophosphatase, mitochondrial, ADP-ribose diphosphatase, ADP-ribose phosphohydrolase, ADP-ribose pyrosphosphatase NUDT9, ADPR-PPase, adenosine diphosphoribose pyrophosphatase, nucleoside diphosphate linked moiety X-type motif 9, nudix (nucleoside diphosphate linked moiety X)-type motif 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BW91
  • Protein Name
    ADP-ribose pyrophosphatase, mitochondrial
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: NUDT9 antibody at 8ug/ml Lane 1: Raji whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Mouse kidney tissue Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 40 kDa
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Figure 1. Western blot analysis of NUDT9 using anti-NUDT9 antibody (A07995). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT9 antigen affinity purified polyclonal antibody (Catalog # A07995) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT9 at approximately 39 kDa. The expected band size for NUDT9 is at 39 kDa.
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