Bio-Connect

Rabbit anti-PFKM Antibody, Affinity Purified

A304-255A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPFKM
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PFKM Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5213
  • Target name
    PFKM
  • Target description
    phosphofructokinase, muscle
  • Target synonyms
    6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; GSD7; PFK1; PFK-1; PFKA; PFK-A; PFKX; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase, polypeptide X; phosphofructokinase-M; phosphohexokinase; PPP1R122; protein phosphatase 1, regulatory subunit 122
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08237
  • Protein Name
    ATP-dependent 6-phosphofructokinase, muscle type
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPFKM
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetPFKM
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
TargetPFKM
  • SizePrice
Product group Antibodies
Assay Genie
TargetPFKM
  • SizePrice
Western Blot analysis of K562 cells using PFKM Polyclonal Antibody
Product group Antibodies
Cusabio
PFKM AntibodyCSB-PA003744
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPFKM
  • SizePrice
Figure 1. Western blot analysis of PFKM using anti-PFKM antibody (A00437-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKM antigen affinity purified polyclonal antibody (Catalog # A00437-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKM at approximately 85 kDa. The expected band size for PFKM is at 85 kDa.
Product group Antibodies
Boster Bio
Anti-PFKM Antibody Picoband(r)A00437-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPFKM
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetPFKM
  • SizePrice
Product group Antibodies
MedChem Express
TargetPFKM
  • SizePrice