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Rabbit anti-PRRC2C Antibody, Affinity Purified

A303-315A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetPRRC2C

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PRRC2C Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23215
  • Target name
    PRRC2C
  • Target description
    proline rich coiled-coil 2C
  • Target synonyms
    BAT2-iso, BAT2D1, BAT2L2, XTP2, protein PRRC2C, BAT2 domain containing 1, BAT2 domain-containing protein 1, HBV X-transactivated gene 2 protein, HBV XAg-transactivated protein 2, HBxAg transactivated protein 2, HLA-B-associated transcript 2-like 2, proline-rich and coiled-coil-containing protein 2C, protein BAT2-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y520
  • Protein Name
    Protein PRRC2C
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRRC2C using anti-PRRC2C antibody (A11762). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRC2C antigen affinity purified polyclonal antibody (Catalog # A11762) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRRC2C at approximately 290,94 kDa. The expected band size for PRRC2C is at 317 kDa.
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