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ProductsBack/Rabbit anti-RCC2 IHC Antibody, Affinity Purified

Rabbit anti-RCC2 IHC Antibody, Affinity Purified

IHC-00155
Bethyl Laboratories
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Equine, Human, Mammals, Monkey, Mouse, Porcine, Primate
TargetRCC2

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-RCC2 IHC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55920
  • Target name
    RCC2
  • Target description
    regulator of chromosome condensation 2
  • Target synonyms
    TD-60, protein RCC2, RCC1-like protein TD-60, epididymis secretory sperm binding protein, telophase disk protein of 60 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P258
  • Protein Name
    Protein RCC2
  • Reactivity
    Canine, Equine, Human, Mammals, Monkey, Mouse, Porcine, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti RCC2 Polyclonal Antibody, Unconjugated (bs-7768R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 56kD. Observed band size: 56kD.
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Figure 1. Western blot analysis of RCC2 using anti-RCC2 antibody (A06203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC2 antigen affinity purified polyclonal antibody (Catalog # A06203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC2 at approximately 56 kDa. The expected band size for RCC2 is at 56 kDa.
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