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ProductsBack/Rabbit anti-Ro60 Antibody, Affinity Purified

Rabbit anti-Ro60 Antibody, Affinity Purified

A303-692A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetRO60
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Ro60 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6738
  • Target name
    RO60
  • Target description
    Ro60, Y RNA binding protein
  • Target synonyms
    RORNP, SSA2, TROVE2, RNA-binding protein RO60, 60 kDa ribonucleoprotein Ro, Ro/SSA 60kDa, Sjogren syndrome antigen A2 (60kD, ribonucleoprotein autoantigen SS-A/Ro), TROVE domain family member 2, gastric cancer multi-drug resistance protein, ro60 autoantigen, sjoegren syndrome type A antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10155
  • Protein Name
    60 kDa SS-A/Ro ribonucleoprotein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TROVE2/SS-A/RO60 using anti-TROVE2/SS-A/RO60 antibody (A03428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TROVE2/SS-A/RO60 antigen affinity purified polyclonal antibody (Catalog # A03428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TROVE2/SS-A/RO60 at approximately 60 kDa. The expected band size for TROVE2/SS-A/RO60 is at 61 kDa.
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