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ProductsBack/Rabbit anti-RPL11 Antibody, Affinity Purified

Rabbit anti-RPL11 Antibody, Affinity Purified

A303-931A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Primate, Rat
TargetRPL11

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-RPL11 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6135
  • Target name
    RPL11
  • Target description
    ribosomal protein L11
  • Target synonyms
    DBA7, GIG34, L11, uL5, large ribosomal subunit protein uL5, 60S ribosomal protein L11, CLL-associated antigen KW-12, cell growth-inhibiting protein 34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62913
  • Protein Name
    Large ribosomal subunit protein uL5
  • Reactivity
    Bovine, Human, Mouse, Primate, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPL11 using anti-RPL11 antibody (A02901-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL11 antigen affinity purified polyclonal antibody (Catalog # A02901-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL11 at approximately 18 kDa. The expected band size for RPL11 is at 18 kDa.
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