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ProductsBack/Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K]

Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K]

A700-205
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetS100A9
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-S100A9 Recombinant Monoclonal Antibody [BLR205K]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR205K
  • Concentration
    25 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6280
  • Target name
    S100A9
  • Target description
    S100 calcium binding protein A9
  • Target synonyms
    60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100-A9, protein S100-A9, MRP-14, calgranulin B, calprotectin L1H subunit, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06702
  • Protein Name
    Protein S100-A9
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9677) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13 kDa. The expected band size for S100A9 is at 13 kDa.
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