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Rabbit anti-SENP1 Antibody, Affinity Purified

A302-927A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetSENP1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SENP1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29843
  • Target name
    SENP1
  • Target description
    SUMO specific peptidase 1
  • Target synonyms
    SuPr-2, sentrin-specific protease 1, SUMO1/sentrin specific peptidase 1, SUMO1/sentrin specific protease 1, sentrin/SUMO-specific protease SENP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0U3
  • Protein Name
    Sentrin-specific protease 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
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