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ProductsBack/Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR239K]

Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR239K]

A700-239
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCdh5
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR239K]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR239K
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID12562
  • Target name
    Cdh5
  • Target description
    cadherin 5
  • Target synonyms
    7B4; 7B4/cadherin-5; AA408225; cadherin-5; Cd144; vascular endothelial cadherin; VE; Vec; VE-C; VEcad; VE-Cad; VE-cadherin; VECD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55284
  • Protein Name
    Cadherin-5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.
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