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ProductsBack/CD144/VE Cadherin Polyclonal Antibody
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.

CD144/VE Cadherin Polyclonal Antibody

BS-0878R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCdh5
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Overview

  • Supplier
    Bioss
  • Product Name
    CD144/VE Cadherin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID12562
  • Target name
    Cdh5
  • Target description
    cadherin 5
  • Target synonyms
    7B4, Cd144, VE-Cad, VECD, VEcad, Vec, cadherin-5, 7B4/cadherin-5, VE-cadherin, vascular endothelial cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55284
  • Protein Name
    Cadherin-5
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang C, Hsu P, Wang D, et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019,21:102078. doi: 10.1016/j.nano.2019.102078
    Read this paper
  • Xia W, Zhang H, Pan Z, et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019,72:211-217. doi: 10.1016/j.intimp.2019.04.009
    Read this paper
  • Bhagwani AR, Hultman S, Farkas D, et al. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension. PLoS One. 2019,14(3):e0213890. doi: 10.1371/journal.pone.0213890
    Read this paper

Datasheet

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Figure 1. Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.
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