Rabbit F1+2 ELISA Kit

Biorbyt

Rabbit F1+2 ELISA Kit

10

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rabbit F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve

ELISA

ELISA

3.13-200 ng/mL

1.39 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2(F1+2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prothrombin Fragment 1+2(F1+2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prothrombin Fragment 1+2(F1+2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prothrombin Fragment 1+2(F1+2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Rabbit

41116158