Western Blot of Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003). Lane M: 3 microl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western Blot of Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003). Lane M: 3 microl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Rabbit IgG F(c) Antibody Fluorescein Conjugated
TA397977
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
Overview
- SupplierOriGene
- Product NameRabbit IgG F(c) Antibody Fluorescein Conjugated
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- HostDonkey
- Scientific DescriptionRabbit IgG F(c) Antibody Fluorescein Conjugated
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203