Rabbit anti Goat IgM (heavy and light chains), conjugated with Biotin

Biorbyt

Rabbit anti Goat IgM (heavy and light chains), conjugated with Biotin

16

Research Use Only

Polyclonal

Biotin

Rabbit

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to goat IgM, heavy and light chains. In immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 250; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

12352203